Blog 6. Learning is experience. Everything else is just information. -Albert Einstein

Week 6 - half of semester one. 

The feedback for Critical Review was given this week. I am quite happy of what I got but reading two of my colleagues' work who got the top marks, I could've done better if more effort was put in. I could've asked more people to critique my work as the feedback gotten from the class was just good work written on it. The extra time given could have used to research more articles regarding the topic rather than just sticking to the article found the first time. However, it is a good learning. Besides, it is the first article critical review that I have done. Having the same assessment in other modules, this will be a great benefit to improve a lot more. Overall, I enjoyed reading the article which made me think scientifically. I am not fund of reading but this module helped me to love and see the benefit of exploring outside the box. It does not only enhance your knowledge but also different abilities like scientific and research abilities which are the big areas that can help me with my thesis for the next semester and also a great advantage in going to industry.

In the lecture, Inclusion Bodies were discussed which I did not understand well. But it has both advantages and disadvantages. It's good to have it as it has potential purification advantage. The reason why I probably don't understand it much is maybe I couldn't think of the advantage of it in our cell line as I haven't came across it in reading articles. However, to get a clearer idea about it, I did my little research. I found out that the key to efficient use of inclusion bodies is to know how to solubilize and refold the protein in high yield. Also, the advantage of E.coli as a cell line was discovered as it is inexpensive, facile to work with, it has success rate and it is scalable. However, it has also disadvantages.  We are still sticking to our cell line which is the vero cell as we found out that we can get it for free. Thus, we can just spend more on a better bioreactor to get a more purified product. 

 Reading: Vaccine - Vero Cells

                 Inclusion Bodies

Prior to the workshop, an article was read that used the same cell line as the one in mind which is vero cell. There were a lot of things learned like it is better to use batch fermentation and then continuous and that perfusion bioreactor has also a lot of advantages. As a group we thought about this and also making it our own way, there are some things to be worked on to overcome the challenges the past researchers faced. Barry gave us a lot of idea on how to improve our method more and how to overcome some of the challenges which will be further discussed by the group in the next group meeting. Also, the draft of infographic was shown. Being the one mainly focused on doing the draft, I must say it is not easy. There are a lot of things to consider. It should be informative at the same time work limited. On the brighter side, I am enjoying this as it is again new to us which is a big advantage in enhancing our skills. 

In conclusion, looking back from the day when I was thinking of dropping the module, I am very grateful that I stayed. This module has a lot of surprises. It may require a lot of work and pressure but there are a lot of skills that has been improved half way through the semester. Also, there are new things that I thought would be hard and impossible to do but turned out really well. The group meetings held each week improved a lot of my communication skills (voicing out my opinion and how I communicate to the team) and of course team work. The infographic, critical review and weekly reading of articles is helping me in my scientific thinking, research abilities and also enhancing my imagination.

Blog 5. Imagination is more important than knowledge. Knowledge is limited. Imagination encircles the world. - Albert Einstein

It has been a really tough week but as last week's motivation quote, it can never be DIFFICULT with HARD WORK.

This week's lecture is all about the different types of bioreactors, which gave us a clear idea of the type of bioreactor that will be suitable for the next big product. The different components, utilities and typical problems were discussed. I find it very beneficial as it explains a lot of the things to consider on choosing the type of bioreactor to use. It also made us think of the possible ways on how to overcome the challenges that may be faced. My group already decided on what to use as researched from the last memo received but the lecture gave us clearer idea on the things that needs to be added and emitted to design the perfect bioreactor for our product. 

The workshop, however, made me realize how my group is doing well for the product we are proposing. This is because when memos are received, we work on it straight away. Ohh yes, we're on the ball! Since we already know what we want to use for our product, we used the time to discuss better ways on how to improve our recent work. As for Barry, it is better to explain it further and so we did and currently reading more articles to broaden our knowledge. However, I figured out that for this module, knowledge is not enough to make things work but most importantly - imagination. A good scientist is to be able to create a product by imagining and figuring things out to make it work. I have attached the articles we've been reading.

This is the current design of bioreactor in mind.

This is now the point where all the informations we have are all connected and it is all going good. I am actually enjoying designing and thinking outside the box as it gives me the feel of working in a real Biopharmaceutical Industry. I look forward to finish our product.

READINGS:

Vero cell line adapted to grow in suspension

Vero cell line which is adapted to grow in suspension, method of obtaining same and viral vaccines

Investigation and Application Progress of Vero Cell Serum-free Culture

Blog 4. "The secret of getting things done is to ACT" - Dante Alighieri

It has been a really tough week with a lot of work that needs to be done. But, to do what needs to be done often takes every ounce of courage that one has. While getting so miserable trying to figure out what to do first and how to do it, I came across this amazing quote "The secret of getting things done is to act" by Dante Alighieri. Yes, that's it, ACT. 

Having decided to read an article about dengue vaccine for critical review, hours spent reading and analysing the paper was all worth it when it was done. That was a work done for critical review and also an added knowledge about the dengue vaccine for e-portfolio. I did exactly what Barry said, to hit two birds in one stone. I felt relieved in the end. 

In the first class, fermentation types, sterilisation and optimization were discussed. A thorough understanding on the different types of fermentation were explained and discussed in class including the advantages and disadvantages. Having discussed all these among our groups made it easier to understand and I felt more informed and also made me think deeper. Ideas for the project came into mind like what's the best type to use for the next big product and the disadvantages that will be a challenge to face. Therefore, to understand it better, after class, I did a research on the types of fermentation and found out that it is best to use batch or continuous for our product. This is because it is suitable for the production of small volume and high potency product like vaccine. However, further reading is still required and I am still going to propose this idea to our next group meeting. I find lectures each week more interesting and slowly seeing the picture of how is it going to be a great help in the industry.

I find the second lecture very discerning. Analysing a critical review of one of my colleagues wasn't easy as I know everybody spent time working on it. However, it's not hard doing the critical review either, if time and effort is much spent. While analysing one's critical review, I constantly compared mine. It gave me a sense of pride as I know having the rubrics as my guide, made my critical review a fair one. On the other hand, I know my one could also be improved. That's why I felt so happy that another week was given to add/revise it. It is indeed true that "Difficult doesn't mean impossible. It simply means that you have to work hard". Hard work does pay off anyway. So, I will work harder to make my critical review not just good but the best.

One BUSY week! 

Blog 3. More everyday foods than you think are fermented.

After learning a good lesson last week which is not to leave anything behind and keeping on track, I am now more organize and confident with each week's tasks. This week's lecture is all about the things to consider when scaling up a bio-processed product, the constraints and basic unit of bio-processing and also sample scenarios were given where suggestions were made which I all find beneficial most especially for starting a small scale for our product. Having to know all the things to consider is definitely a great help in the making of our proposed product. For me, the most significant part of the lecture is the Fermentation. It engrossed me more due to the fact that we use it every day. However, when asked to list fermented products, it seemed like everybody has the same idea and I felt like there must be more. Therefore, I decided to consult our dearest friend Mr. Google. I found out that most fermented products are in Asia like the "puto", made of semi-glutinous rice fermented in earthen jars, which is Philippine's famous delicacy. I also found out the benefits of fermented products which tend to break down pesticides store in fats.

As for the second memo from Bioplus, as a group, we came up with a lot of ideas for the next big product. But, we focused on the product that is much needed. We all decided to make a vaccine and then we asked ourselves, "Vaccine for what?". We searched for infectious diseases that have no vaccine yet. In the end, we all agreed to make a vaccine for dengue which can treat all the four strains DENV-1, DENV-2, DENV-3 and DENV-4. However, that is a big challenge for us which we will work on. The presentation of products of different groups just like The Dragons' Den was also a challenge. I was very impressed of all the ideas each group came up with. Also, I am more than proud of my group. It feels like we are already in a real industry proposing a product which is about to make a difference in the world. The challenges we're facing on our product is really making us come out of our own shell and be creative yet scientific thinkers. 

The benefit of the module is now all coming together. I am slowly seeing a light and it only encourages me more to take the path to Bio-pharmaceutical Industry. At first, I hate reading but this module encouraged me how important it is to be updated and to enhance my knowledge. I am now fund of reading articles that are beneficial for our proposed product. I am currently reading articles to find out candidate cells for our product. 

For the coming week, the only thing in my mind is KEEP ON TRACK and ENJOY. It's time to read more articles and do the Critical Review.